| Expression System | CHO |
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| Protein Tag | C-FLAG |
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| Purity | > 95% by SDS-PAGE under reducing conditions |
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| Endotoxin | < 1 EU per ug by the LAL method |
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| Activity | Positive |
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| Conjugate | Unconjugated |
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| Species | Human |
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| Accession Number | P50897-1 |
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| Protein Sequence | Asp28-Gly306 |
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| Molecular Weight | The protein has a predicted molecular weight of 32.6 kDa. The protein migrates at approximately 35-40 kDa on SDS-PAGE with DTT-reduced condition. |
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| Formulation | 20mM Phosphate, 150mM NaCl, pH 6.5, 10%(v/v) Glycerol (0.2 µm filtered) |
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| Concentration | 25µg size is bottled at 0.2mg/mL concentration. 100 µg and 500 µg size is bottled at lot specific concentration. |
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| Purification | Affinity Ni-NTA |
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| Applications | ELISA |
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| Format | Liquid |
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| Reconstitution | NA |
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| Shipping Condition | Cold Pack |
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| Storage/Shelf Life | An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. This stock solution should be aliquoted and stored at -70°C to minimize the freeze/thaw cycles. |
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| Background Information | The human PPT1 protein, also known as palmitoyl-protein thioesterase 1, is a lysosomal enzyme that plays a crucial role in the breakdown of proteins. Specifically, it removes thioester-linked fatty acyl groups, like palmitate, from cysteine residues in proteins, aiding in their degradation within lysosomes. This process is essential for proper cellular function and development. |
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